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Deshmukh, P and Markande, S and Fandade, V and Ramtirtha, Y and Madhusudhan, MS and Joseph, J (2021) The miRISC component AGO2 has multiple binding sites for Nup358 SUMO-interacting motif. Biochemical and Biophysical Research Communications, 556. pp. 45-52.

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Micro-RNA mediated suppression of mRNA translation represents a major regulatory mode of post-transcriptional gene expression. Recently, the nucleoporin Nup358 was shown to interact with AGO protein, a key component of miRNA-induced silencing complex (miRISC), and facilitate the coupling of miRISC with target mRNA. Previous results suggested that SUMO-interacting motifs (SIMs) present on Nup358 mediate interaction with AGO protein. Here we show that Nup358-SIM has multiple interacting regions on AGO2, specifically within the N, PAZ and MID domains, with an affinity comparable to SIM-SUMO1 interaction. The study also unraveled specific residues involved in the interaction of AGO2 with miRNA-loading components such as Dicer and HSP90. Collectively, the results support the conclusion that multiple SIMs contribute to the association of Nup358 with AGO2.

Item Type: Article
Subjects: Cell Biology
Depositing User: Mr. Rameshwar Nema
Date Deposited: 20 Nov 2021 12:49
Last Modified: 20 Nov 2021 12:49
URI: http://nccs.sciencecentral.in/id/eprint/1022

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