Piprode, V. and Singh, K. and Kumar, A. and Josh, SR. and Wani, MR. (2021) IL-3 inhibits rat osteoclast differentiation induced by TNF-a and other pro-osteoclastogenic cytokines. Journal of Bioscience, 46 (63). pp. 1-12.

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Abstract

IL-3, a haematopoiesis regulatory factor, has previously been shown to inhibit both mouse and human osteoclast differentiation and bone resorption. Here, the role of rat IL-3 on rat osteoclast differentiation was evaluated to address whether the inhibitory action of IL-3 on osteoclastogenesis is conserved in various species. It was observed that IL-3 inhibited rat osteoclast differentiation induced by both TNF-α and receptor activator of NF-κB ligand (RANKL). TNF-α is known to induce bone loss in postmenopausal osteoporotic women and it also synergise with many pro-osteoclastogenic cytokines to cause huge pathological bone loss. Importantly, it was found that rat IL-3 inhibits the synergistic action of TNF-α with RANKL and IL-1β, TGF-β1 and TGF-β3. IL-3 downregulates the TNF-α-induced nuclear translocation of NF-κB-p65 and c-fos without affecting c-jun. Interestingly, we observed that IL-3 also inhibits osteoclast differentiation in vivo in rats induced by TNF-α. All these results suggest that inhibitory action of IL-3 on osteoclastogenesis is conserved in various species including mice, rats and humans. Thus, our results clearly indicate that IL-3 has therapeutic potential to treat pathological bone loss in important skeletal diseases.

Item Type:	Article
Subjects:	Cell Biology
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	23 Nov 2021 07:46
Last Modified:	23 Nov 2021 07:46
URI:	http://nccs.sciencecentral.in/id/eprint/1055

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