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Mote, RD and Laxmikant , VS and Singh, SB and Tiwari, M and Singh, H and Srivastava, J and Tripathi , V and Seshadri, V and Majumdar, A and Subramanyam, D (2021) A cost-effective and efficient approach for generating and assembling reagents for conducting real-time PCR. Journal of Bioscience, 46 (4). p. 109.

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Real-time PCR is a widely used technique for quantification of gene expression. However, commercially available kits for real-time PCR are very expensive. The ongoing coronavirus pandemic has severely hampered the economy in a number of developing countries, resulting in a reduction in available research funding. The fallout of this will result in limiting educational institutes and small enterprises from using cutting edge biological techniques such as real-time PCR. Here, we report a cost-effective approach for preparing and assembling cDNA synthesis and real-time PCR mastermixes with similar efficiencies as commercially available kits. Our results thus demonstrate an alternative to commercially available kits.

Item Type: Article
Subjects: Cell Biology
Depositing User: Mr. Rameshwar Nema
Date Deposited: 12 Oct 2022 05:50
Last Modified: 12 Oct 2022 05:50
URI: http://nccs.sciencecentral.in/id/eprint/1139

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