Assaiya , A and Bhar, S and Kumar, J (2022) Chapter 19 : Advances in Sample Preparation and Data Processing for Single-particle Cryo-Electron Microscopy. In: Advances in Protein Molecular and Structural Biology Methods. Methods in Molecular and Structural Biology. Methods in Molecular and Structural Biology . Academic Press Elsevier, pp. 291-309. ISBN 978-032-39-0264-9

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Abstract

Single-particle cryoelectron microscopy (cryoEM) is the most popular method currently for high-resolution structure determination of purified macromolecules or their complexes in solution. The requirement of a lower amount of protein samples and the removal of bottlenecks such as crystallization and size limit that are limitations of X-ray crystallography and nuclear magnetic resonance, the other two commonly used methods for structure determination, has contributed significantly to the widespread use of cryoEM. Besides, the availability of user-friendly, intuitive, and free software packages for academic researchers has further boosted its usage. Single-particle cryoEM has provided a new impetus to the structural biology of complex biological molecules that can now be examined with unparalleled ease and efficiency, illuminating the molecular structure and the dynamics of biologically relevant molecules at high resolution. This chapter provides an overview of the various steps of structure determination via single-particle analysis, primarily focusing on sample preparation and data processing. In addition, we summarize the AI/ML-based tools developed for the various steps of data processing that have not only improved the quality of 3D reconstructions but, importantly, also raise hopes of the complete automation of the data processing pipelines.

Item Type:	Book Section
Subjects:	Structural Biology
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	12 Oct 2022 07:43
Last Modified:	12 Oct 2022 07:43
URI:	http://nccs.sciencecentral.in/id/eprint/1152

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