Bhattacharyya, S and Mote , RD and Freimer, JW and Singh, SB and Arumugam, S and Narayana , YV and Rajan, R and Subramanyam , D (2022) Cell–cell adhesions in embryonic stem cells regulate the stability and transcriptional activity of β-catenin. FEBS Letters, 596 (13). pp. 1647-1660.

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Abstract

E-cadherin (CDH1) is involved in maintaining cell–cell adhesions in embryonic stem cells (ESCs). However, its function in the context of cell fate decisions is largely unknown. Using mouse ESCs (mESCs), we demonstrate that E-cadherin and β-catenin interact at the membrane and continue to do so upon internalization within the cell. Cdh1−/− mESCs failed to form tight colonies, with altered differentiation, marker expression and retention of pluripotency factors during differentiation. Interestingly, Cdh1−/− mESCs showed dramatically reduced β-catenin levels. Transcriptional profiling of Cdh1−/− mESCs displayed a significant alteration in the expression of a subset of β-catenin targets in a cell state- and GSK3β-dependent manner. Our findings hint at hitherto unknown roles played by E-cadherin in regulating the activity of β-catenin in ESCs.

Item Type:	Article
Subjects:	Stem Cell Biology
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	19 Oct 2022 12:04
Last Modified:	19 Oct 2022 12:04
URI:	http://nccs.sciencecentral.in/id/eprint/1184

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