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Chavan, S and Khuperkar, D and Lonare, A and Panigrahi , S and Bellare, J and Rapole , S and Seshadri , V and Joseph, J (2022) Ran GTPase links nucleo-cytoplasmic transport to the recruitment of cargoes into small extracellular vesicles. Cellular and Molecular Life Sciences, 79. p. 392.

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Small extracellular vesicle (sEV)-mediated intercellular communication regulates multiple aspects of growth and development in multicellular organisms. However, the mechanism underlying cargo recruitment into sEVs is currently unclear. We show that the key nucleo-cytoplasmic transport (NCT) protein—RanGTPase, in its GTP-bound form (RanGTP), is enriched in sEVs secreted by mammalian cells. This recruitment of RanGTP into sEVs depends on the export receptor CRM1 (also called XPO1). The recruitment of GAPDH, a candidate cargo protein, into sEVs is regulated by the RanGTP–CRM1axis in a nuclear export signal (NES)-dependent manner. Perturbation of NCT through overexpression or depletion of nuclear transport components affected the recruitment of Ran, CRM1 and GAPDH into sEVs. Our studies, thus, suggest a link between NCT, particularly the Ran–CRM1 axis, and recruitment of NES-containing cargoes into the sEVs. Collectively, these findings implicate RanGTPase as a link between NCT and sEV mediated intercellular communication.

Item Type: Article
Subjects: Cell Biology
Depositing User: Mr. Rameshwar Nema
Date Deposited: 20 Oct 2022 05:57
Last Modified: 20 Oct 2022 05:57
URI: http://nccs.sciencecentral.in/id/eprint/1186

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