Varankar, SS and Hari, K and Kartika , S and Bapat, SA and Jolly, MK (2022) Cell geometry distinguishes migration-associated heterogeneity in two-dimensional systems. Computational and System Oncology, 2 (3). E1041.

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Abstract

In vitro migration assays are a cornerstone of cell biology and have found extensive utility in research. Over the past decade, several variations of the two-dimensional (2D) migration assay have improved our understanding of this fundamental process. However, the ability of these approaches to capture the functional heterogeneity during migration and their accessibility to inexperienced users has been limited. We downloaded published time-lapse 2D cell migration data sets and subjected them to feature extraction with the Fiji software. We used the “Analyze Particles” tool to extract 10 cell geometry features (CGFs), which were grouped into “shape,” “size,” and “position” descriptors. Next, we defined the migratory status of cells using the “MTrack2” plugin. All data obtained from Fiji were further subjected to rigorous statistical analysis with R version 4.0.2. We observed consistent associative trends between size and shape descriptors and validated our observations across four independent data sets. We used these descriptors to identify and characterize “nonmigrator (NM)” and “migrator (M)” subsets. Statistical analysis allowed us to identify considerable heterogeneity in the NM subset. Interestingly, differences in 2D-packing appeared to affect CGF trends and heterogeneity within the migratory subsets. We developed an analytical pipeline using open source tools, to identify and morphologically characterize functional migrat

Item Type:	Article
Subjects:	Cancer Biology
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	20 Oct 2022 07:15
Last Modified:	20 Oct 2022 07:15
URI:	http://nccs.sciencecentral.in/id/eprint/1191

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