Mohandasan , R and Thakare, M and Sunke, S and Iqbal, FM and Sridharan , M and Das, G (2022) Enhanced olfactory memory detection in trap-design Y-mazes allows the study of imperceptible memory traces in Drosophila. Learning Memory, 29 (10). pp. 355-366.

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Abstract

The neural basis of behavior is identified by systematically manipulating the activity of specific neurons and screening for loss or gain of phenotype. Therefore, robust, high-scoring behavioral assays are necessary for determining the neural circuits of novel behaviors. We report a simple Y-maze design for Drosophila olfactory learning and memory assay. Memory scores in our Y-mazes are considerably better and longer-lasting than scores obtained with commonly used T-mazes. Our results suggest that trapping flies to an odor choice in a Y-maze could improve scores. We postulated that the improved scores could reveal previously undetectable memory traces, enabling the study of underlying neural mechanisms. Indeed, we identified unreported protein synthesis-dependent long-term memories (LTMs), reinforced by ingestion of (1) an aversive compound and (2) a sweet but nonnutritious sugar, both 24 h after training. We also used Y-mazes to probe how using a greater reward may change memory dynamics. Our findings predict that a greater sugar reward may extend existing memory traces or reinforce additional novel ones.

Item Type:	Article
Subjects:	Cell Biology
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	03 Jan 2023 07:01
Last Modified:	03 Jan 2023 07:01
URI:	http://nccs.sciencecentral.in/id/eprint/1224

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