Khuperkar , D. and Helen, M. and Magre , I. and Joseph , J. (2015) Inter-Cellular Transport of Ran GTPase. PLoS One. pp. 1-15.

Preview

Text 26.Dr. Joseph J. (PLOS One)-c@author.pdf - Published Version Download (1181Kb) | Preview

Abstract

Ran, a member of the Ras-GTPase superfamily, has a well-established role in regulating the transport of macromolecules across the nuclear envelope (NE). Ran has also been implicated in mitosis, cell cycle progression, and NE formation. Over-expression of Ran is associated with various cancers, although the molecular mechanism underlying this phenomenon is unclear. Serendipitously, we found that Ran possesses the ability to move from cell-to-cell when transiently expressed in mammalian cells. Moreover, we show that the inter-cellular transport of Ran is GTP-dependent. Importantly, Ran displays a similar distribution pattern in the recipient cells as that in the donor cell and co-localizes with the Ran binding protein Nup358 (also called RanBP2). Interestingly, leptomycin B, an inhibitor of CRM1-mediated export, or siRNA mediated depletion of CRM1, significantly impaired the inter-cellular transport of Ran, suggesting a function for CRM1 in this process. These novel findings indicate a possible role for Ran beyond nucleo-cytoplasmic transport, with potential implications in inter-cellular communication and cancers.

Item Type:	Article
Additional Information:	This is Open Access Journal (for full text click above weblink)
Subjects:	Cell Biology
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	12 May 2015 09:49
Last Modified:	18 May 2015 08:47
URI:	http://nccs.sciencecentral.in/id/eprint/176

Actions (login required)

View Item