Sarkar , R and Sarkar , K and Singh, N.B and Singh , Y.M and Mitra, D. and Chakrabarti , S (2012) Emergence of a unique recombinant form of HIV-1 from Manipur (India). Journal of Clinical Virology, 55. pp. 274-277.

Text 28. Dr. Mande (Cell Stress & Chaper - Author copy.pdf - Accepted Version Restricted to Registered users only until June 2016. Download (621Kb) | Request a copy

Abstract

Background: The AIDS epidemic in Manipur, India, manifests unique features, having co-circulation of B and C HIV-1 subtypes along with recombinant forms. Manipur has the highest incidence of HIV-1 infection compared to the other states of India, but limited information is available regarding the fulllength sequence of HIV-1 recombinants. Objectives: To characterize the near full-length genome of a novel recombinant HIV-1 strain from an injecting drug user of Manipur. Study design: Viral RNA, extracted from the plasma of a male injecting drug user aged 35, was diagnosed with HIV-1 infection. Near full-length genome was amplified by polymerase chain reaction using primer walking approach. Phylogenetic relationships were determined with neighbor-joining trees. The recombination break points were detected using boot scan and Simplot analyses. Results: This recombinant predominantly had subtype C genome and exhibited mosaic structures with subtype B insertions at three different positions of HIV-1 genome. Simplot analysis of near full-length genome sequence from the recombinant HIV-1 strain, MAN86 exhibited similarity with the sequence of C.IN.93.93IN905 in its subtype C backbone, while the subtype B insertions showed resemblance with the sequence of B.TH.99.99TH C1416. Conclusions: This study confirms the presence of a unique recombinant HIV-1 strain, emerging as a result of recombination between HIV-1 strains from India and Thailand.

Item Type:	Article
Additional Information:	Annual Report 2012-2013 (Sr. No. 5)
Subjects:	Cancer Biology
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	09 Apr 2015 06:19
Last Modified:	02 Jul 2015 09:15
URI:	http://nccs.sciencecentral.in/id/eprint/20

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