Mandlik , V. and Gurav , M. and Singh, S. (2015) Regulatory dynamics of network architecture and function in Tristable genetic circuit of Leishmania: A Mathematical biology Approach. J Biomol Struct Dyn. .

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Abstract

The emerging field of synthetic biology has led to the design of tailor-made synthetic circuits for several therapeutic applications. Biological networks can be re-programmed by designing synthetic circuits that modulate the expression of target proteins. IPCS (Inositol phosphorylceramide synthase) has been an attractive target in the sphingolipid metabolism of the parasite 'Leishmania'. In the present study, we have constructed a tristable circuit for the IPCS protein. The circuit has been validated and its long term behaviour has been assessed. The robustness and evolvability of the circuit has been estimated by using evolutionary algorithms. The tristable synthetic circuit has been specifically designed to improve the rate of production of Phosphatidylcholine: ceramide cholinephosphotransferase 4 (SLS4 protein). Site specific delivery of the circuit into the parasite infected macrophages could serve as a possible therapeutic intervention of the infectious disease 'Leishmaniasis'.

Item Type:	Article
Subjects:	Structural Biology
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	01 Sep 2015 10:31
Last Modified:	01 Sep 2015 10:32
URI:	http://nccs.sciencecentral.in/id/eprint/202

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