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Shrivastava , S. and Trivedi , J. and Mitra , D. (2016) Gene expression profiling reveals Nef induced deregulation of lipid metabolism in HIV-1 infected T cells. Biochemical and Biophysical Research Communications, 472 (1). pp. 169-174.

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Human Immunodeficiency Virus-1 (HIV-1) encodes a 27 kDa Negative Factor or Nef protein, which is increasingly proving to be a misnomer. Nef seems to be crucial for AIDS progression as individuals infected with nef-deleted strain of HIV were reported to become Long Term Non Progressors (LTNP). These findings necessitate tracing of Nef's footprint on landscape of cellular transcriptome favoring HIV-1 pathogenesis. We have tried to explore effect of Nef on cellular gene expression profile in conjunction with rest of HIV-1 proteins. Our results show that 237 genes are differentially regulated due to the presence of Nef during infection, which belong to several broad categories like "signaling", "apoptosis", "transcription" and "lipid metabolism" in gene ontology analysis. Furthermore, our results show that Nef causes disruption of lipid content in HIV-1 infected T cells. Molecular inhibitors of lipid metabolism like Atorvastatin and Ranolazine were found to have profound effect on wild type virus as compared to nef-deleted HIV-1. Thus our results suggest that interference in lipid metabolism is a potential mechanism through which Nef contributes in enhancing HIV-1 pathogenesis.

Item Type: Article
Additional Information: This is Open Access article for full text please click above weblink
Subjects: Cancer Biology
Depositing User: Mr. Rameshwar Nema
Date Deposited: 24 May 2016 08:32
Last Modified: 24 May 2016 08:34
URI: http://nccs.sciencecentral.in/id/eprint/235

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