Jiang, X. and Peery , A. and Hall , A.B. and Sharma, A. and Chen, X.G. and Waterhouse , R.M. and Komissarov , A. and Riehle , M.M. and Shouche , Y. and Sharakhova , M.V. and Lawson , D. and Pakpour , N. and Arensburger , P. and Davidson , V.L. and Eiglmeier , K. and Emrich , S and George , P. and Kennedy , R.C. and Mane , S.P. and Maslen , G. and Oringanje , C. and Qi Y, Y. and Settlage , R. (2014) Genome analysis of a major urban malaria vector mosquito, Anopheles stephensi. Gnome Biology , 15 (9). p. 459.

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Abstract

BACKGROUND: Anopheles stephensi is the key vector of malaria throughout the Indian subcontinent and Middle East and an emerging model for molecular and genetic studies of mosquito-parasite interactions. The type form of the species is responsible for the majority of urban malaria transmission across its range. RESULTS: Here, we report the genome sequence and annotation of the Indian strain of the type form of An. stephensi. The 221 Mb genome assembly represents more than 92% of the entire genome and was produced using a combination of 454, Illumina, and PacBio sequencing. Physical mapping assigned 62% of the genome onto chromosomes, enabling chromosome-based analysis. Comparisons between An. stephensi and An. gambiae reveal that the rate of gene order reshuffling on the X chromosome was three times higher than that on the autosomes. An. stephensi has more heterochromatin in pericentric regions but less repetitive DNA in chromosome arms than An. gambiae. We also identify a number of Y-chromosome contigs and BACs. Interspersed repeats constitute 7.1% of the assembled genome while LTR retrotransposons alone comprise more than 49% of the Y contigs. RNA-seq analyses provide new insights into mosquito innate immunity, development, and sexual dimorphism.

Item Type:	Article
Additional Information:	This is Open Access article for full text click above weblink
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	27 May 2016 03:52
Last Modified:	27 May 2016 03:52
URI:	http://nccs.sciencecentral.in/id/eprint/248

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