Meyerson JR1, , J.R. and Rao P1, P. and Kumar J2, , J. and Chittori S2, , S and Banerjee S1, , S and Pierso, J. and Mayer, M.L. and Subramaniam , S. (2014) Self-assembled monolayers improve protein distribution on holey carbon cryo-EM supports. Sci. Report, 4. p. 7084.

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Abstract

Poor partitioning of macromolecules into the holes of holey carbon support grids frequently limits structural determination by single particle cryo-electron microscopy (cryo-EM). Here, we present a method to deposit, on gold-coated carbon grids, a self-assembled monolayer whose surface properties can be controlled by chemical modification. We demonstrate the utility of this approach to drive partitioning of ionotropic glutamate receptors into the holes, thereby enabling 3D structural analysis using cryo-EM methods.

Item Type:	Article
Additional Information:	THIS IS OPEN ACCESS ARTICLE FOR FULL TEXT CLICK ABOVE WEBLINK
Subjects:	Cell Biology
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	27 May 2016 08:57
Last Modified:	27 May 2016 08:57
URI:	http://nccs.sciencecentral.in/id/eprint/255

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