Bhandyopadhyay , S. and Mahapatra , S.K. and Chowdhury , P.B. and Jha , M.K. and Das, S. and Halder , K. and Majumdar , S.B. and Saha , B. and Majumder , S. (2015) Toll-like receptor 2 targeted rectification of impaired CD8+ T-cell functions in experimental Leishmania donovani infection reinstates host protection. PLoS One. 0142800.

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Abstract

Leishmania donovani, a protozoan parasite, causes the disease visceral leishmanisis (VL), characterized by inappropriate CD8+ T-cell activation. Therefore, we examined whether the Toll-like Receptor 2 (TLR2) ligand Ara-LAM, a cell wall glycolipid from non-pathogenic Mycobacterium smegmatis, would restore CD8+ T-cell function during VL. We observed that by efficient upregulation of TLR2 signaling-mediated NF-κB translocation and MAPK signaling in CD8+ T-cells (CD25+CD28+IL-12R+IFN-γR+), Ara-LAM triggered signaling resulted in the activation of T-bet, which in turn, induced transcription favourable histone modification at the IFN-γ, perforin, granzyme-B promoter regions in CD8+ T-cells. Thus, we conclude that Ara-LAM induced efficient activation of effector CD8+ T-cells by upregulating the expression of IFN-γ, perforin and granzyme-B in an NF-κB and MAPK induced T-bet dependent manner in VL.

Item Type:	Article
Additional Information:	This is Open Access article
Subjects:	Infection and Immunity
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	13 Dec 2016 07:33
Last Modified:	22 Feb 2021 11:39
URI:	http://nccs.sciencecentral.in/id/eprint/305

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