Kasas , S. and Radotic, K. and Longo , G. and Saha , B. and Alonso-Sarduy , L. and Dietler, G and Roduit , C. (2013) A Universal Fluid Cell for the Imaging of Biological Specimens in the Atomic Force Microscope. MICROSCOPY RESEARCH AND TECHNIQUE 76:357–363 (2013), 76. pp. 357-363.

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Abstract

Recently, atomic force microscope (AFM) manufacturers have begun producing instruments specifically designed to image biological specimens. In most instances, they are integrated with an inverted optical microscope, which permits concurrent optical and AFM imaging. An important component of the set-up is the imaging chamber, whose design determines the nature of the experiments that can be conducted. Many different imaging chamber designs are available, usually designed to optimize a single parameter, such as the dimensions of the substrate or the volume of fluid that can be used throughout the experiment. In this report, we present a universal fluid cell, which simultaneously optimizes all of the parameters that are important for the imaging of biological specimens in the AFM. This novel imaging chamber has been successfully tested using mammalian, plant, and microbial cells.

Item Type:	Article
Subjects:	Infection and Immunity
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	13 Apr 2015 06:11
Last Modified:	03 Jul 2015 05:20
URI:	http://nccs.sciencecentral.in/id/eprint/52

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