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Fernandes , S. and Tembe, S. and Shinde, P. and Melinkeri , S. and Kale , V. and Limaye , L. (2018) Establishment of human iPSC line NCCSi003-A from CD34+cells of peripheral blood collected during apheresis of healthy donor from Indian ethnicity. Stem Cell Research, 27. pp. 1-4.

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We present generation of iPSCs from CD34+ cells isolated from peripheral blood, collected during apheresis of a healthy female individual. We nucleofected the CD34+cells by episomal vectors containing Oct4, Sox2, L-Myc, Lin28, Klf4 and p53DD (dominant negative mutation in p53). The resultant colonies showed cobble-stone appearance and stained positive for alkaline phosphatase. The colonies demonstrated presence of pluripotency markers by immunofluorescence, flow-cytometry and PCR. The plasmids were lost from cells subsequently during passages as assessed by PCR. Karyotype analysis demonstrated a stable genome. The cells had capability to differentiate to cells from all three-germ lineages in vitro.

Item Type: Article
Subjects: Stem Cell Biology
Depositing User: Mr. Rameshwar Nema
Date Deposited: 11 Dec 2018 11:38
Last Modified: 13 Dec 2021 11:09
URI: http://nccs.sciencecentral.in/id/eprint/533

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