Nirujogi , R.S. and Pawar , H. and Renuse, S. and Kumar, P. and Chavan , S. and Sathe , G. and Sharma , J. and Khobragade , S. and Pande , J. and Modak , B. and Prasad , T.S.K. and Harsha , H.C and Patole, M.S. and Pande , A. (2014) Moving from unsequenced to sequenced genome: Reanalysis of the proteome of Leishmania donovani☆. Journal of Proteomics, 97 (31). pp. 48-61. ISSN Volume 97, 31 January 2014, Pages 48–61

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Abstract

The kinetoplastid protozoan parasite, Leishmania donovani, is the causative agent of kala azar or visceral leishmaniasis. Kala azar is a severe form of leishmaniasis that is fatal in the majority of untreated cases. Studies on proteomic analysis of L. donovani thus far have been carried out using homology-based identification based on related Leishmania species (L. infantum, L. major and L. braziliensis) whose genomes have been sequenced. Recently, the genome of L. donovani was fully sequenced and the data became publicly available. We took advantage of the availability of its genomic sequence to carry out a more accurate proteogenomic analysis of L. donovani proteome using our previously generated dataset. This resulted in identification of 17,504 unique peptides upon database-dependent search against the annotated proteins in L. donovani. These peptides were assigned to 3999 unique proteins in L. donovani. 2296 proteins were identified in both the life stages of L. donovani, while 613 and 1090 proteins were identified only from amastigote and promastigote stages, respectively. The proteomic data was also searched against six-frame translated L. donovani genome, which led to 255 genome search-specific peptides (GSSPs) resulting in identification of 20 novel genes and correction of 40 existing gene models in L. donovani.

Item Type:	Article
Additional Information:	This is Online Free article (for full text click above weblink)
Subjects:	Bioinformatics and Proteomics
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	13 Apr 2015 06:41
Last Modified:	03 Jul 2015 05:30
URI:	http://nccs.sciencecentral.in/id/eprint/55

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