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Mol, M. and Kosey, D. and Boppana, R. and Singh , S. (2018) Transcription Factor Target Gene Network governs the Logical Abstraction Analysis of the Synthetic Circuit in Leishmaniasis. Scientific Report, 8 (1). p. 3464.

49.Dr. Singh S. (Sci Rep) open access.pdf

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With the advent of synthetic biology in medicine many synthetic or engineered proteins have made their way to therapeutics and diagnostics. In this paper, the downstream gene network of CD14-TNF-EGFR pathway in leishmaniasis, a tropical disease, is reconstructed. Network analysis showed that NFkB links the signaling and gene network, used as a point of intervention through a synthetic circuit embedded within the negative autoregulatory feedback loop. A chimeric protein kinase C (PKC) is incorporated in the synthetic circuit, under the transcriptional regulation of Lac repressor and IPTG, as an inducer. The chimeric PKC_ζα via IκKb phosphorylation activates NFκB, and modulates the gene expression from an anti-inflammatory to a pro-inflammatory phenotype in in vitro L. major infected macrophage model. This is the first ever report of a synthetic device construction in leishmania.

Item Type: Article
Subjects: Bioinformatics and Proteomics
Depositing User: Mr. Rameshwar Nema
Date Deposited: 14 Dec 2018 12:00
Last Modified: 19 Feb 2021 06:23
URI: http://nccs.sciencecentral.in/id/eprint/553

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