Behera, P. and Mahapatra, M. and Seuylemezian, A. and Vaishampayan, P. and Ramana, V.V. and Joseph, N. and Joshi, A. and Shouche, Y. and Suar, M. and Pattnaik, A.K. and Rastogi, G. (2018) Taxonomic description anddraft genome of Pseudomonas sediminis sp. nov., isolated from the rhizosphericsediment of Phragmites karka. Journal of Microbiolgy, 56 (7). pp. 458-466.

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Abstract

The taxonomic position of a Gram-stain-negative, rod-shaped bacterial strain, designated PI11T, isolated from the rhizo-spheric sediment of Phragmites karka was characterized using a polyphasic approach. Strain PI11T could grow optimally at 1.0% NaCl concentration with pH 7.0 at 30°C and was posi-tive for oxidase and catalase but negative for hydrolysis of starch, casein, and esculin ferric citrate. Phylogenetic analy-sis of 16S rRNA gene sequences indicated that the strain PI11T belonged to the genus Pseudomonas sharing the highest sequence similarities with Pseudomonas indoloxydans JCM 14246T (99.72%), followed by, Pseudomonas oleovorans subsp. oleovorans DSM 1045T (99.29%), Pseudomonas toyotomien-sis JCM 15604T (99.15%), Pseudomonas chengduensis DSM 26382T (99.08%), Pseudomonas oleovorans subsp. lubricantis DSM 21016T (99.08%), and Pseudomonas alcaliphila JCM 10630T (99.01%). Experimental DNA-DNA relatedness be-tween strain PI11T and P. indoloxydans JCM 14246T was 49.4%. The draft genome of strain PI11T consisted of 4,884,839 bp. Average nucleotide identity between the genome of strain PI11T and other closely related type strains ranged between 77.25–90.74%. The polar lipid pattern comprised of phos-phatidylglycerol, diphosphatidylglycerol, and phosphatidyl-choline. The major (> 10%) cellular fatty acids were C18:1 ω6c/ω7c, C16:1 ω6c/ω7c, and C16:0. The DNA G + C content of strain PI11T was 62.4 mol%. Based on the results of poly-phasic analysis, strain PI11T was delineated from other clo-sely related type strains. It is proposed that strain PI11T rep-

Item Type:	Article
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	10 Feb 2020 11:51
Last Modified:	10 Feb 2020 11:51
URI:	http://nccs.sciencecentral.in/id/eprint/596

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