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Chopra, K. and Bawaria, S. and Chauhan, R. (2019) Evolutionary divergence of the nuclear porecomplex from fungi to metazoans. Protein Science, 28 (3). pp. 571-586.

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Nuclear pore complex (NPC) is the largest multimeric protein assembly of the eukaryoticcell, which mediates the nucleocytoplasmic transport. The constituent proteins of this assembly (nucleo-porins) are present in varying copy numbers to give a size from ~ 60 MDa (yeast) to 112 MDa (human)and share common ancestry with other membrane-associated complexes such as COPI/COPII and thusshare the same structural folds. However, the nucleoporins across species exhibit very low percentagesequence similarity and this reflects in their distinct secondary structure and domain organization. Weemployed thorough sequence and phylogenetic analysis guided from structure-based alignments of allthe nucleoporins from fungi to metazoans to understand the evolution of NPC. Through evolutionarypressure analysis on various nucleoporins, we deduced that these proteins are under differential selec-tion pressure and hence the homologous interacting partners do not complement each other in thein vitro pull-down assay. The super tree analysis of all nucleoporins taken together illustrates divergentevolution of nucleoporins and notably, the degree of divergence is more apparent in higher order organ-isms as compared to lower species. Overall, our results support the hypothesis that the protein–proteininteractions in such large multimeric assemblies are species specific in nature and hence their structureand function should also be studied in an organism-specificmanner.

Item Type: Article
Depositing User: Mr. Rameshwar Nema
Date Deposited: 11 Feb 2020 04:59
Last Modified: 11 Feb 2020 05:15
URI: http://nccs.sciencecentral.in/id/eprint/601

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