Fernandes, S. and Tembe, S. and Singh, S. and Vardhan, S. and Nair, V. and Kale, V. and Limaye, L. (2019) . Development and characterization of human iPSC line NCCSi004-A from umbilicalcord blood (UCB) derived CD34(+)cells obtained from donor belonging to Indianethnic population. Stem Cell Research, 26 (35). p. 101392.

Text 19. Dr. Limaye (Stem Cell Res.) open access.pdf Restricted to Repository staff only Download (1006Kb) | Request a copy

Abstract

Here we report the reprogramming of CD34+ cells obtained from UCB of a healthy donor female child belonging to the Indian ethnic population. These CD34+cells were subjected to nucleofection for delivery of episomal vectors expressing Oct4, Sox2, L-Myc, Lin28, Klf4 and p53DD (negative mutation in p53). The iPSC colonies expressed pluripotency markers as detected by PCR, immunofluorescence and flow-cytometry. The removal of plasmid was confirmed by its absence in cells at higher passages. Karyotype analysis revealed a stable genome. The property of in vitro differentiation to tri-lineage was confirmed by expression of markers by immunofluorescence.

Item Type:	Article
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	11 Feb 2020 08:49
Last Modified:	09 Dec 2021 11:26
URI:	http://nccs.sciencecentral.in/id/eprint/609

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