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Fernandes, S. and Tembe, S. and Singh, S. and Vardhan, S. and Nair, V. and Kale, V. and Limaye, L. (2019) . Development and characterization of human iPSC line NCCSi004-A from umbilicalcord blood (UCB) derived CD34(+)cells obtained from donor belonging to Indianethnic population. Stem Cell Research, 26 (35). p. 101392.

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Here we report the reprogramming of CD34+ cells obtained from UCB of a healthy donor female child belonging to the Indian ethnic population. These CD34+cells were subjected to nucleofection for delivery of episomal vectors expressing Oct4, Sox2, L-Myc, Lin28, Klf4 and p53DD (negative mutation in p53). The iPSC colonies expressed pluripotency markers as detected by PCR, immunofluorescence and flow-cytometry. The removal of plasmid was confirmed by its absence in cells at higher passages. Karyotype analysis revealed a stable genome. The property of in vitro differentiation to tri-lineage was confirmed by expression of markers by immunofluorescence.

Item Type: Article
Depositing User: Mr. Rameshwar Nema
Date Deposited: 11 Feb 2020 08:49
Last Modified: 09 Dec 2021 11:26
URI: http://nccs.sciencecentral.in/id/eprint/609

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