Kalra, R.S. and Bapat , S.A. (2013) Expression Proteomics Predicts Loss of RXR-c during Progression of Epithelial Ovarian Cancer. PLoS One , 8 (8). e70398-e70398.

Text 58-1. Dr. Bapat S(PLoS ONE) Open Access- c@author.pdf - Published Version Restricted to Registered users only Download (933Kb) | Request a copy

Abstract

The process of cellular transformation involves cascades of molecular changes that are modulated through altered epigenetic, transcription, post-translational and protein regulatory networks. Thus, identification of transformationassociated protein alterations can provide an insight into major regulatory pathways activated during disease progression. In the present protein expression profiling approach, we identified differential sets of proteins in a two-dimensional gel electrophoresis screen of a serous ovarian adenocarcinoma progression model. Function-based categorization of the proteins exclusively associated with pre-transformed cells identified four cellular processes of which RXR-c is known to modulate cellular differentiation and apoptosis. We thus probed the functional relevance of RXR-c expression and signaling in these two pathways during tumor progression. RXR-c expression was observed to modulate cellular differentiation and apoptosis in steady-state pre-transformed cells. Interestingly, retinoid treatment was found to enhance RXR-c expression in transformed cells and sensitize them towards apoptosis in vitro, and also reduce growth of xenografts derived from transformed cells. Our findings emphasize that loss of RXR-c levels appears to provide mechanistic benefits to transformed cells towards the acquisition of resistance to apoptosis hallmark of cancer, while effective retinoid treatment may present a viable approach towards sensitization of tumor cells to apoptosis through induction of RXR-c expression.

Item Type:	Article
Subjects:	Cancer Biology
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	13 Apr 2015 08:54
Last Modified:	09 Jul 2015 06:48
URI:	http://nccs.sciencecentral.in/id/eprint/62

Actions (login required)

View Item