Markande, A.R. and Vemuluri, V.R. and Shouche, Y.S. and Nerurkar, A.S. (2018) Characterization of Solibacillus silvestris strain AM1 that produces amyloid bioemulsifier. Journal of Basic Microbiology, 58 (6). pp. 523-531.

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Abstract

Solibacillus silvestris AM1 was the first strain from the genus to be reported for the production of a functional amyloid and its potential use as a surface active agent, a thermostable glycoprotein amyloid bioemulsifier BE-AM1 capable of influencing environment and biofilm formation. Phylogenetic analysis based on 16S rRNA gene, molecular characterization studies on the basis of DNA-DNA hybridization and chemotaxonomic fatty acid methyl ester (FAME) analysis showed that S. silvestris AM1 as a strain matches with the type strain S. silvestris HR3-23. But strain AM1 differs from the type strain HR3-23 in carbon substrate utilization studies along with amyloid bioemulsifier production ability with potential industrial and environmental applications. S. silvestris AM1 exhibited bioemulsifier production at wide range of factors like pH and NaCl concentrations, while temperature influenced the bioemulsifier production indirectly (since it affected the growth). Bioemulsifier production was observed even at oligotrophic conditions (0.5mg/ml) seen usually in its native environmant. In this study, we have characterized the amyloid producing S. silvestris AM1 taxonomically and also analyzed 16S rDNA of 103 sequences of Solibacillus sp. available, which indicated the possibility of new species in this genus and can be studied for industrially and environmentally important biomolecules.

Item Type:	Article
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	13 Feb 2020 06:27
Last Modified:	13 Feb 2020 06:27
URI:	http://nccs.sciencecentral.in/id/eprint/630

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