Singh, J. and Ambi, U.B. (2019) A Comparative Whole Genome Sequence Analysis Leads toIdentification of Repeat-Associated Evolutionarily Conserved miRNAs in Bombyxmori (Lepidoptera: Bombycidae). Journal of Insect Science, 19 (3).

Text 58-1. Dr. Jyoti Singh (INSPIRED SCIENTIST) J.Insect Sci. - open access article.pdf Restricted to Repository staff only Download (1086Kb) | Request a copy

Abstract

MicroRNAs (miRNAs) are evolutionarily conserved small noncoding RNAs, which play important regulatory roles in various biological processes. In this study, we have developed a computational approach for detecting conserved miRNAs based on comparison of whole genome sequences of closely related species by considering various key features of experimentally validated miRNAs. By applying this approach, we have identified 34 new miRNAs from Bombyx mori (L.), which are also conserved in Drosophila melanogaster (Diptera: Drosophilidae) and Anopheles gambiae (Diptera: Culicidae). Most of these miRNAs were associated with repeat region of the genome. We did an expression analysis of the 34 newly predicted miRNAs and found that 30 of these miRNAs are expressing in different tissues of B. mori. Besides, we have also predicted the putative targets of these miRNAs in B. mori based on several known characteristic features of miRNA::mRNA duplexes and found that these targets include diverse range of functions, suggesting multiple layers of gene regulation of various important biological processes.

Item Type:	Article
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	21 Feb 2020 04:01
Last Modified:	08 Dec 2021 11:12
URI:	http://nccs.sciencecentral.in/id/eprint/722

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