Khan, M. and Zende, S. and Vaidyanath, A. and Avatade, R. and Shiras, A. (2019) Generation of two induced pluripotent stem cell lines NCCSi005A and NCCSi006A from CD4(+)T cells of healthyindividuals of Indian origin. Stem Cell Research, 39.

Text 27-1. Dr. Shiras A. (Stem Cell Res.) open access.pdf Restricted to Repository staff only Download (1303Kb) | Request a copy

Abstract

Human induced pluripotent stem cell-lines (iPSCs) of Indian origin NCCSi005A and NCCSi006A were established by reprogramming of CD4+T cells, isolated from the peripheral blood mononuclear cells (PBMCs) of two healthy female donors. Reprogramming was achieved using integration free, Sendai viral vector system expressing cocktail of transcription factors KOS, hc-MYC and hKLF4. Both the established cell-lines showed alkaline phosphatase activity, expressed stemness markers, exhibited normal female karyotype and displayed potential for tri-lineage differentiation. These two CD4+T cells derived cell-lines represent valuable resource as control iPSC cell lines of Indian origin.

Item Type:	Article
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	21 Feb 2020 06:30
Last Modified:	06 Dec 2021 11:57
URI:	http://nccs.sciencecentral.in/id/eprint/739

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