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Khan, M. and Vaidyanath, A. and Suresh, D. and Vaishnav, B. and Kakrani, A.L. and Patil, S. and Shiras, A. (2019) Generation and characterization of three clones (NCCSi007A, NCCSi007B andNCCSi007C) of an integration free induced luripotent Stem Cell line from apatient with alcoholic liver cirrhosis of Indian ethnicity. Stem Cell Research., 42.

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Abstract

Three induced pluripotent stem cells (iPSC) clones NCCSi007-A, NCCSi007-B and NCCSi007-C were generated from CD4+T cells of a 38 years old male patient suffering from liver cirrhosis- alcoholic and minimal hepatic encephalopathy of Indian origin. The CD4+T cells of the patient were reprogrammed using integration free, Sendai viral vector system. Each of the three iPSC clones showed high alkaline phosphatase (ALP) activity, expressed pluripotency markers OCT4, SOX2, NANOG, KLF4, SSEA-4, TRA-1–60, showed normal male karyotype (46, XY) and exhibited multi-lineage differentiation.

Item Type: Article
Depositing User: Mr. Rameshwar Nema
Date Deposited: 25 Feb 2020 05:14
Last Modified: 06 Dec 2021 11:55
URI: http://nccs.sciencecentral.in/id/eprint/777

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