Kumar, A. and Lekha, R. and Mhaske, S.T. and Pote, S.T. and Behera, S. and Mishra, G.C. and Wani, M.R. (2020) IL-3 receptor expression on activated human Th cells is regulated by IL-4; and IL-3 synergies with IL-4 to enhance Th2 cell differentiation. The Journal of Immunology.

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Abstract

IL-3, a cytokine secreted by activated T lymphocytes, is known to regulate the proliferation, survival, and differentiation of hematopoietic cells. However, the role of IL-3 in regulation of T cell functions is not fully delineated. Previously, we have reported that IL-3 plays an important role in development of regulatory T cells in mice. In this study, we investigated the regulation of IL-3R expression on human Th cells and also examined the role of IL-3 in effector functions of these cells. We found that human peripheral blood Th cells in resting state do not show surface expression of IL-3R; however, its expression was observed at transcript and intracellular protein levels. The functional IL-3R expression on the surface was seen only after antigenic stimulation. When naive Th cells were activated in the presence of various cytokines, we found that IL-4 significantly increases the surface expression of IL-3R and also increases the number of IL-3R+ Th cells. Interestingly, IL-3R+ cells exhibit a Th2 cell–like phenotype and show high GATA-3 expression. Moreover, Th2 cells in presence of IL-3 show increased expression of type 2 effector cytokines, such as IL-4, IL-5, and IL-13. Furthermore, IL-3R expressing and IL-3–secreting Th cells were high in house dust mite–allergic patients. Thus, to our knowledge, we provide the first evidence that the expression of IL-3R on activated human Th cells is modulated by IL-4, and IL-3 regulates the effector functions of Th2 cells. Our results suggest that IL-3 may play an important role in regulating allergic immune responses.

Item Type:	Article
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	25 Feb 2020 06:19
Last Modified:	25 Feb 2020 06:19
URI:	http://nccs.sciencecentral.in/id/eprint/786

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