Bag, P. and Ojha , D. and Mukherjee , H. and Halder , U.C. and Mondal , S. and Biswas , A. and Sharon , A. and Van Kaer , L. and Chakrabarty , S. and Das , G. and Mitra, D. and Chattopadhyay, D. (2014) A dihydro-pyrido-indole potently inhibits HSV-1 infection by interfering the viral immediate early transcriptional events. Antiviral Research, 105. pp. 126-134.

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Abstract

In our continued quest for identifying novel molecules from ethnomedicinal source we have isolated an alkaloid 7-methoxy-1-methyl-4,9-dihydro-3H-pyrido[3,4-b]indole, also known as Harmaline (HM), from an ethnomedicinal herb Ophiorrhiza nicobarica. The compound exhibited a potent anti-HSV-1 activity against both wild type and clinical isolates of HSV-1. Further we demonstrated that HM did not interfere in viral entry but the recruitment of lysine-specific demethylase-1 (LSD1) and the binding of immediateearly (IE) complex on ICP0 promoter. This leads to the suppression of viral IE gene synthesis and thereby the reduced expression of ICP4 and ICP27. Moreover, HM at its virucidal concentration is nontoxic and reduced virus yields in cutaneously infected Balb/C mice. Thus, the interference in the binding of IE complex, a decisive factor for HSV lytic cycle or latency by HM reveals an interesting target for developing non-nucleotide antiherpetic agent with different mode of action than Acyclovir.

Item Type:	Article
Additional Information:	Annual Report 2013-2014 (Sr.No.4)
Subjects:	Cancer Biology
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	15 Apr 2015 10:05
Last Modified:	08 Jul 2015 09:06
URI:	http://nccs.sciencecentral.in/id/eprint/84

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