Tembe , S and Fernandes, S and Khan, N and Melinkeri , S and Kale , V and Limaye, L (2020) Establishment of human iPSC line from patient of Indian ethnicity carrying homozygous CD8/9 (+G) beta thalassemia mutation. Stem Cell Research, 44. p. 101772.

Text 21. Dr. Limaye LS. (Stem Cell Res.) open access.pdf Restricted to Repository staff only Download (1399Kb) | Request a copy

Abstract

This study shows generation of iPSCs from peripheral blood mononuclear cells (PBMNCs) of a male patient having homozygous CD 8/9 (+G) beta thalassemia (major) mutation. Cells were nucleofected with episomal vectors containing Oct4, Sox2, L-Myc, Lin28, Klf4 and p53DD (dominant negative p53 mutation). Cell line exhibited presence of pluripotency markers by immunofluorescence, flow-cytometry and PCR. The plasmids were lost from cells by subsequent passages, observed by PCR. Karyotype analysis demonstrated a stable genome. The cells had capability to differentiate into three-germ lineages in vitro. This iPSC line can be used as a tool for drug design and gene therapy studies.

Item Type:	Article
Subjects:	Stem Cell Biology
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	19 Jan 2021 06:54
Last Modified:	29 Nov 2021 10:37
URI:	http://nccs.sciencecentral.in/id/eprint/859

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