Vaidyanath , A and Khan , M and Vaishnav , B and Kakrani , AL and Patil , S and Shiras , A (2020) Developmentand characterization of two independent integration free iPSC clones NCCSi010-Aand NCCSi010-B from a patient with alcoholic liver cirrhosis of Indianethnicity. Stem Cell Ressearch. , 47. p. 10191. ISSN Stem Cell Ressearch. 2020. Volume 47, August 2020, 101911doi: 10.1016/j.scr.2020.101911.

Text 44.-1 Dr. Shiras A (Stem Cell Res.) open access.pdf Restricted to Repository staff only Download (3434Kb) | Request a copy

Abstract

We generated two human induced pluripotent stem cell-line (iPSC) clones, NCCSi010-A and NCCSi010-B, from a 32-year-old alcoholic cirrhosis patient with minimal hepatic encephalopathy of Indian origin by reprogramming his CD4+ T cells with integration free Sendai viral vector system. The generated iPSC clones showed high alkaline phosphatase activity, expressed pluripotency markers, possessed potential for multi-lineage differentiation and exhibited a normal karyotype (46, XY). These two-patient specific iPSC clones of alcoholic liver cirrhosis can potentially serve as models for disease modeling, drug development and organoid generation (Shah and Bataller, 2016).

Item Type:	Article
Subjects:	Stem Cell Biology
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	27 Apr 2021 13:09
Last Modified:	29 Nov 2021 10:40
URI:	http://nccs.sciencecentral.in/id/eprint/952

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