Choudhury, A. and Mohammad, T. and Samarth, N. and Hussain, A. and Rehman , MT. and Islam, A. and Alajmi , MF. and Singh, S. and Hassan, MI. (2021) Structural genomics approach to investigate deleteriousimpact of nsSNPs in conserved telomere maintenance component 1. Scientific Report., 11 (1). p. 10202.

Text 27-1. Dr. Singh S. (Sci Rep) open access.pdf Restricted to Repository staff only Download (6Mb) | Request a copy

Abstract

Conserved telomere maintenance component 1 (CTC1) is an important component of the CST (CTC1-STN1-TEN1) complex, involved in maintaining the stability of telomeric DNA. Several non-synonymous single-nucleotide polymorphisms (nsSNPs) in CTC1 have been reported to cause Coats plus syndrome and Dyskeratosis congenital diseases. Here, we have performed sequence and structure analyses of nsSNPs of CTC1 using state-of-the-art computational methods. The structure-based study focuses on the C-terminal OB-fold region of CTC1. There are 11 pathogenic mutations identified, and detailed structural analyses were performed. These mutations cause a significant disruption of noncovalent interactions, which may be a possible reason for CTC1 instability and consequent diseases. To see the impact of such mutations on the protein conformation, all-atom molecular dynamics (MD) simulations of CTC1-wild-type (WT) and two of the selected mutations, R806C and R806L for 200 ns, were carried out. A significant conformational change in the structure of the R806C mutant was observed. This study provides a valuable direction to understand the molecular basis of CTC1 dysfunction in disease progression, including Coats plus syndrome.

Item Type:	Article
Subjects:	Insect Molecular Biology
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	22 Nov 2021 08:42
Last Modified:	26 Nov 2021 11:34
URI:	http://nccs.sciencecentral.in/id/eprint/1039

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