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Kumar , MR. and Jani , K. and Parvathi , JR. and Thomas, BM. and Raja , SSS. and Pandey , A. and Sharma, A. (2022) Bacterial diversity of geochemically distinct hot springs located in Maharashtra, India. Archives of Microbiology, 204. p. 110.

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Bacterial diversity of four thermally different hot springs of Ratnagiri district, Maharashtra, India, was investigated using culture-dependent and culture-independent approaches. A total of 144 bacterial cultures were isolated and identified using MALDI-TOF MS (matrix-assisted laser desorption ionization-time of flight mass spectrometry) and 16S rRNA gene sequencing. Culture-independent analysis by Ion Torrent sequencing targeting the V3 region of the 16S rRNA gene revealed the predominance of Firmicutes across all the hot springs, followed by Chloroflexi, Bacteroidetes, Cyanobacteria, Proteobacteria, Armatimonadetes, Actinobacteria, Nitrospirae, Acidobacteria, and Deinococcus–Thermus, with subtle differences in their abundance. At the lower taxonomic rank of genus, we noted the prevalence of Acinetobacter followed by Clostridium, Planomicrobium, Bacillus, Streptomyces, and Leptolyngbya. Metagenomics imputation using in silico approach revealed divergence in the metabolic capabilities of bacterial communities along the thermal gradient of host springs, with site TS (63 °C) featuring the abundant functional gene families.

Item Type: Article
Subjects: Insect Molecular Biology
Depositing User: Mr. Rameshwar Nema
Date Deposited: 14 Feb 2022 07:53
Last Modified: 14 Feb 2022 07:54
URI: http://nccs.sciencecentral.in/id/eprint/1110

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