Ranebennur, H and Kirdat , K and Tiwarekar, B and Rawat, K and Chalam, VC and Solanke, AU and Yadav, R and Singh, K and Sathe, S and Yadav, A and Rao, GP (2022) Draft genome sequence of 'Candidatus Phytoplasma australasia', strain SS02 associated with sesame phyllody disease. 3 Biotech, 12 (5). p. 107.

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Abstract

‘Candidatus Phytoplasma’ is an uncultivated, intracellular bacterial plant pathogen transmitted by phloem-feeding insect vectors. Among the group of phytoplasmas, the Peanut Witches' Broom or 16SrII group of phytoplasmas associated with various diseases cause severe crop losses every year in India. The ‘Ca. Phytoplasma sp.’ strain SS02 was associated with phyllody disease of sesame plants collected from New Delhi. The genome sequence of strain SS02 was obtained using its genomic DNA enrichment and hybrid assembly of sequences generated on Illumina and Oxford Nanopore Technologies MinION platforms. The hybrid assembly strategy generated a draft genome with 60 contigs totaling 553,228 bp of length with more than 400 × depth coverage and 95.21% of the estimated completeness. The SS02 genome draft sequence contains 465 protein-coding genes, 17 tRNA genes, and 3 rRNA genes. The availability of this draft genome also provided a foundation for genome-scale genotypic analyses.

Item Type:	Article
Additional Information:	1. Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, Pusa Campus, New Delhi, 110 012, India. 2. National Centre for Cell Science, NCCS Complex, SSPU Campus, Ganeshkhind, Pune, 411 021, India. (CA) 3. ICAR-National Bureau of Plant Genetic Resources, Pusa Campus, New Delhi-110 012, India. 4. Tuljaram Chaturchand College, Baramati, Maharashtra, 413 102, India. 5
Subjects:	Insect Molecular Biology
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	18 Oct 2022 12:06
Last Modified:	18 Oct 2022 12:06
URI:	http://nccs.sciencecentral.in/id/eprint/1175

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