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Chauhan, NS and Joseph, N and Shaligram, S and Chavan, N and Joshi , A and Dhotre , D and Lodha, T and Shouche, YS (2022) Paenibacillus oleatilyticus sp. nov., isolated from soil. Archives of Microbiology. pp. 204-516.

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Abstract

A gram-stain-negative, endo-spore forming, facultatively anaerobic, motile, rod-shaped bacterial strain SM69T, isolated from soil samples of Rohtak, Haryana, India was characterized using polyphasic approach. White colonies were 2–3 mm, in diameter and growth occurred between 20 and 55 °C, pH 6.0–10.0 with 0–2.0% (w/v) NaCl. Based on 16S rRNA gene sequence similarity the strain is placed in the genus Paenibacillus as it is closely related to ‘Paenibacillus tyrfis MSt1T’ (99.7%) and P. elgii SD17T (99.6%). The cell wall peptidoglycan contained meso-diaminopimelic acid. The dominant fatty acids included anteiso-C15: 0 (50%), C16: 0 (12%) and anteiso-C17: 0 (10%). Major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The size of the draft genome was 7,848,017 bp, with 53.1% G+C content. dDDH (51.6%) and ANI (93.5%) of strain SM69T with its close relatives indicates that it represents a novel species, for which the name Paenibacillus oleatilyticus sp. nov. (Type strain SM69T = MCC 3064T = JCM 33981T = KACC 21649T) is proposed.

Item Type: Article
Subjects: Insect Molecular Biology
Depositing User: Mr. Rameshwar Nema
Date Deposited: 20 Oct 2022 06:57
Last Modified: 20 Oct 2022 06:57
URI: http://nccs.sciencecentral.in/id/eprint/1189

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