Apte , S and Bhutda , S and Ghosh, S and Sharma, K and Santra, MK and Neill, DR and Banerjee , A (2023) An innate pathogen sensing strategy involving ubiquitination of bacterial surface proteins. Science Advances, 9 (12). p. 1851.

Full text not available from this repository. (Request a copy)

Abstract

Sensing of pathogens by ubiquitination is a critical arm of cellular immunity. However, universal ubiquitination targets on microbes remain unidentified. Here, using in vitro, ex vivo, and in vivo studies, we identify the first protein-based ubiquitination substrates on phylogenetically diverse bacteria by unveiling a strategy that uses recognition of degron-like motifs. Such motifs form a new class of intra-cytosolic pathogen-associated molecular patterns (PAMPs). Their incorporation enabled recognition of nonubiquitin targets by host ubiquitin ligases. We find that SCFFBW7 E3 ligase, supported by the regulatory kinase, glycogen synthase kinase 3β, is crucial for effective pathogen detection and clearance. This provides a mechanistic explanation for enhanced risk of infections in patients with chronic lymphocytic leukemia bearing mutations in F-box and WD repeat domain containing 7 protein. We conclude that exploitation of this generic pathogen sensing strategy allows conservation of host resources and boosts antimicrobial immunity.

Item Type:	Article
Additional Information:	1.Indian Institute of Technology Bombay, Maharashtra, India. 2.University of Liverpool, L69 7BE Liverpool, UK. 3.National Centre for Cell Science, Maharashtra, India. 4.CSIR-Centre for Cellular and Molecular Biology, Telangana, India. 5.University of Leicester, Leicester, UK. 6. Indian Institute of Science, Bengaluru , Karnataka, India. 7. Center for Functional Genomics of Microbes, University of Greifswald, D-17487 Greifswald, Germany.
Subjects:	Insect Molecular Biology
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	18 Apr 2023 10:52
Last Modified:	18 Apr 2023 10:52
URI:	http://nccs.sciencecentral.in/id/eprint/1253

Actions (login required)

View Item