Pawar , K.D and Banskar , S. and Rane , S.D and Charan , S.S and Kulkarni , G.J and Sawant , S.S and Patole , M.S. and Shouche , Y.S. (2012) Bacterial diversity in different regions of gastrointestinal tract of Giant African Snail (Achatina fulica). Microbiology Open. pp. 415-426.

Text 20-1.Dr. Shouche (Microbiology Open ) Open Access -c@author.pdf - Published Version Restricted to Registered users only Download (200Kb) | Request a copy

Abstract

The gastrointestinal (GI) tract of invasive land snail Achatina fulica is known to harbor metabolically active bacterial communities. In this study, we assessed the bacterial diversity in the different regions of GI tract of Giant African snail, A. fulica by culture-independent and culture-dependent methods. Five 16S rRNA gene libraries from different regions of GI tract of active snails indicated that sequences affiliated to phylum c-Proteobacteria dominated the esophagus, crop, intestine, and rectum libraries, whereas sequences affiliated to Tenericutes dominated the stomach library. On phylogenetic analysis, 30, 27, 9, 27, and 25 operational taxonomic units (OTUs) from esophagus, crop, stomach, intestine, and rectum libraries were identified, respectively. Estimations of the total bacterial diversity covered along with environmental cluster analysis showed highest bacterial diversity in the esophagus and lowest in the stomach. Thirty-three distinct bacterial isolates were obtained, which belonged to 12 genera of two major bacterial phyla namely c-Proteobacteria and Firmicutes. Among these, Lactococcus lactis and Kurthia gibsonii were the dominant bacteria present in all GI tract regions. Quantitative real-time polymerase chain reaction (qPCR) analysis indicated significant differences in bacterial load in different GI tract regions of active and estivating snails. The difference in the bacterial load between the intestines of active and estivating snail was maximum. Principal component analysis (PCA) of terminal restriction fragment length polymorphism suggested that bacterial community structure changes only in intestine when snail enters estivation state.

Item Type:	Article
Subjects:	Insect Molecular Biology
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	09 Apr 2015 07:07
Last Modified:	09 Jul 2015 04:19
URI:	http://nccs.sciencecentral.in/id/eprint/23

Actions (login required)

View Item