Polkade, A.V. and Ramana, V.V. and Joshi , A. and Pardesi, L. and Shouche, Y.S. (2015) Rufibacter immobilis sp. nov., isolated from a high-altitude saline. Int J Syst Evol Microbiol, 65 (5). pp. 1592-1597.

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Abstract

Two pinkish-red, Gram-stain-negative, non-motile aerobic bacterial strains (MCC P1(T) and MCC P2), capable of growing at low temperatures (15 °C), were isolated from water of a saline lake located in the western Himalayas of India. The strains were capable of growth in the presence of 0-2.0% NaCl and at pH 6.5-9.0. Phylogenetic analysis based on 16S rRNA gene sequences revealed the closest similarity of 96.3% to the type strain of the only species of the genus Rufibacter , Rufibacter tibetensis CCTCC AB 208084(T). Strains MCC P1(T) and MCC P2 shared 99.0% 16S rRNA gene sequence similarity and 88.6% DNA-DNA relatedness. The major cellular fatty acids were iso-C15 : 0, C17 : 1ω6c, summed feature 3 (C16 : 1ω6c/C16 : 1ω7c) and summed feature 4 (anteiso-C17 : 1 B/iso-C17 : 1 I). Predominant polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The respiratory quinone was MK-7. The DNA G+C content of the strains was 52.6-52.8 mol%. Based on morphological, physiological, chemotaxonomical and molecular characteristics, strains MCC P1(T) and MCC P2 represent a novel species of the genus Rufibacter , for which the name Rufibacter immobilis sp. nov. is proposed. The type strain is MCC P1(T) ( =MCC 2268(T) =CCTCC AB 2013351(T)).

Item Type:	Article
Subjects:	Insect Molecular Biology
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	21 Dec 2016 09:16
Last Modified:	21 Dec 2016 09:16
URI:	http://nccs.sciencecentral.in/id/eprint/360

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