Prakash , O. and Prakash , P.K. and Kulkarni, G.L. and Mahale , K.N. and Shouche , Y.S. (2014) Technicalities and Glitches of Terminal Restriction Fragment Length Polymorphism (T-RFLP). Indian Journal of Microbiology, 54 (3). pp. 255-261.

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Abstract

Terminal restriction fragment length polymorphism (T-RFLP) is a rapid, robust, inexpensive and simple tool for microbial community profiling. Methods used for DNA extraction, PCR amplification and digestion of amplified products have a considerable impact on the results of T-RFLP. Pitfalls of the method skew the similarity analysis and compromise its high throughput ability. Despite a high throughput method of data generation, data analysis is still in its infancy and needs more attention. Current article highlights the limitations of the methods used for data generation and analysis. It also provides an overview of the recent methodological developments in T-RFLP which will assist the readers in obtaining real and authentic profiles of the microbial communities under consideration while eluding the inherent biases and technical difficulties.

Item Type:	Article
Subjects:	Insect Molecular Biology
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	22 Dec 2016 02:13
Last Modified:	22 Dec 2016 02:13
URI:	http://nccs.sciencecentral.in/id/eprint/362

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