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Sharma , A. and Dhar, S.K. and Prakash, O. and Vemuluri, V.R. and Thite , V. and Shouche , Y.S. (2014) Description of Domibacillus indicus sp. nov., isolated from ocean sediments and emended description of the genus Domibacillus. Int J Syst Evol Microbiol. 2014 Sep;64(Pt 9):3010-5. doi: 10.1099/ijs.0.064295-0. Epub 2014 Jun 6, 64 (9). pp. 3010-3015.

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Abstract

A novel Gram-stain-positive, spore-forming, aerobic, non-motile, rod-shaped bacterium designated strain SD111(T) that forms red-pigmented colonies was isolated from a marine sediment sample (collected from 5 m depth) from Lakshadweep, India. Strain SD111(T) grew well on seawater agar at pH 6-10 (optimum pH 7.5±0.2). It showed maximum (97.6 %) 16S rRNA gene sequence similarity and formed a monophyletic clade with Domibacillus robiginosus WS 4628(T) ( = DSM 25058(T)). The genomic DNA G+C content was 37.4 mol% and the strain showed 37.7 % DNA-DNA relatedness to D. robiginosus DSM 25058(T). The major fatty acids were anteiso-C15 : 0, C16 : 0, iso-C15 : 0 and iso-C16 : 0 and MK-6 was the predominant quinone. The polar lipid profile of strain SD111(T) consisted of unidentified phospholipids (PL1 and PL2), phosphatidylglycerol (PG) and diphosphatidylglycerol (DPG). The cell wall contained meso-diaminopimelic acid and the peptidoglycan was of A1γ type. Glucose and ribose were detected as major cell-wall sugars. Results from polyphasic studies indicated that SD111(T) represents a novel species of the genus Domibacillus for which the name Domibacillus indicus sp. nov. is proposed. The type strain is SD111(T) ( = MCC 2255(T) = DSM 28032(T)).

Item Type: Article
Subjects: Insect Molecular Biology
Depositing User: Mr. Rameshwar Nema
Date Deposited: 22 Dec 2016 02:40
Last Modified: 22 Dec 2016 02:40
URI: http://nccs.sciencecentral.in/id/eprint/365

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