Prakash , O and Nimonkar , Y and Shouche , Y.S. (2013) Practice and prospects of microbial preservation. FEMS Microbiol Lett , 339. pp. 1-9.

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Abstract

A growing interest in culturable diversity has required microbiologists to think seriously about microbial preservation. In addition to the isolation and cultivation of pure strains, adequate preservation without changes in morphological, physiological and genetic traits is necessary. This review consolidates different methods used for preservation of microorganisms with an emphasis on cryopreservation and lyophilization. The critical points of cryopreservation and lyophilization are highlighted to explain how several extrinsic and intrinsic factors affect the cell survival and recovery during the process of long-term preservation. Factors responsible for alteration in genotypic and phenotypic integrity of cultures during preservation and methods used for their evaluation have been incorporated. We emphasize the importance of depositories and highlight their current funding status. Future areas for preservation research, including cell dormancy, ecosystem and community level preservation and the effects of the viable but non-culturable state on post-preservation recovery of the cells are also discussed. Introduction Extensive research in microbiology has revealed that almost all ecosystem services, including biogeochemical cycling of materials, degradation of pollutants, wastewater treatment and production of oxygen, are mediated by microbial metabolism (Hayat et al., 2010; Senni et al., 2011). Microbes are the backbone of modern biotech industries and are utilized for generation of bioenergy and biofuels (Stahl & Wagner, 2006; Bhardwaj & Garg, 2012). They are a natural source of novel therapeutics, used as bio-pesticides and bio-fertilizers, and for maintaining the sustainability of the environment (Zaidi et al., 2009; Hayat et al., 2010). Despite providing valuable ecosystem services, microbes also act as causative agents of diseases and affect human health and hygiene (Arrigo, 2005; Lal et al., 2010; Singh et al., 2010; Kostka et al., 2011). Increasing awareness about culturable diversity and the development of modern cultivation approaches is constantly increasing the numbers of new and previously uncultivated taxa of microorganisms in culture collections (Zengler et al., 2002; Leadbetter, 2003; Giovannoni & Stingl, 2007; Alain & Querellou, 2009; Pace, 2009). Cultivation and characterization of microorganisms alone is not adequate without preservation techniques that do not alter the mor

Item Type:	Article
Subjects:	Insect Molecular Biology
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	13 Apr 2015 04:20
Last Modified:	02 Jul 2015 10:44
URI:	http://nccs.sciencecentral.in/id/eprint/41

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