Choudhury, S.R. and More , T.H. and Chattopadhyay, R. and Lodh, I. and Ray, C.D. and Bose, G. and Sarkar, H.S. and Chakravarthy , B. and Rapole, S. and Chaudhury K., K. (2017) Polycystic ovary syndrome in Indian women: a mass spectrometry based serum metabolomics approach. Metabolomics, 13 (115).

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Abstract

Introduction Polycystic ovary syndrome (PCOS) is a complex, heterogeneous endocrinological disorder with uncertain pathogenesis and is very common in women of reproductive age. There are few reports of utilizing metabolomics approach to understand the complex pathophysiology of PCOS. However, excluding one previous NMR-based metabolomics study, none of the study was conducted in Indian population. Objective The study aims to compare the serum metabolomic profile of PCOS women with controls from the Eastern region of India. Methods PCOS women (n = 35) and healthy control women (n = 30) undergoing tubal ligation were recruited for this study. Serum metabolic profiles were generated using liquid chromatography–tandem mass spectrometry (LC-MS/MS) and gas chromatography–mass spectrometry (GC-MS). Multivariate statistical analysis was applied to spectral data obtained from both the LC-MS/MS and GC/MS. Results Nine metabolites were identified to be most significantly dysregulated in sera of PCOS women; however, few other identified metabolites were also altered but with lesser significance. Amongst these metabolites, riboflavin, sucrose, adenine and N-acetyl glycine, phosphoric acid and cortisol were down-regulated, whereas, thymine, cystathionine, and phenylalanine were up-regulated in PCOS when compared with controls. The observed changes in metabolite expression suggested alterations in aminoacyl-tRNA biosynthesis, metabolism of nitrogen, alanine-aspartate-glutamate, galactose, glycine-serine-threonine, and pyrimidine-purine among several metabolic pathways possibly implicated in these PCOS women.

Item Type:	Article
Subjects:	Insect Molecular Biology
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	11 Dec 2018 08:00
Last Modified:	11 Dec 2018 08:00
URI:	http://nccs.sciencecentral.in/id/eprint/529

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