Dar, MA and Pawar, KD and Rajput, BP and Rahi, P and Pandit, RS (2019) Purification of a cellulase from cellulolytic gut bacterium, Bacillus tequilensis G9 and its evaluation for valorization of agro-wastes into added value byproducts. Biocatalysis and Agricultural Biotechnology (20). p. 101219.

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Abstract

Bioconversion of lignocellulosic (LC) biomass is the most promising alternative to rapidly dwindling fossil fuels and greenhouse gas emissions. In view of this objective, Bacillus tequilensis strain G9 was characterized and explored for purification of its cellulase. The B. tequilensis G9 was found to convert LC substrates into reducing sugars and showed significant activity of 130.75 IU/ml extract on grass straw (GS). The X-ray diffraction (XRD) pattern and fourier transform infra-red (FTIR) spectroscopic analyses of the hydrolyzed GS revealed increased crystallinity index and exclusion of bands at wave numbers 898, 980 and 1543 cm−1 which represent digestion of cellulose content by bacterium. Liquid chromatography mass spectrometric (LC-MS) analysis revealed the identity of purified enzyme as Endoglucanase. The observed molecular mass of the purified cellulase was 43 kDa, optimally functional at acidic pH of 5.0 and temperature 40 °C. The cellulase activity was remarkably enhanced by DTT, CO2+, Ca2+ and Zn2+ ions while inhibited by SDS, EDTA and Pb. Additionally, the cellulase hydrolyzed CMC into fermentable sugars like glucose and galactose which can be readily used in metabolic as well as industrial processes. Further, the co-culturing of B. tequilensis G9 with yeast resulted in the production of ethanol from LC waste. The above mentioned cellulolytic repertoire of B. tequilensis G9 signpost its potential for biotechnological applications.

Item Type:	Article
Subjects:	Insect Molecular Biology
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	30 Aug 2020 09:08
Last Modified:	30 Aug 2020 09:08
URI:	http://nccs.sciencecentral.in/id/eprint/819

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