Sawant , A.A. and Tanpure , A.A. and Mukherjee , P.P. and Athavale , S. and Galande , S. (2015) A versatile toolbox for posttranscriptional chemical labeling and imaging of RNA. Nucleic Acid Research.

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Abstract

Cellular RNA labeling strategies based on bioorthogonal chemical reactions are much less developed in comparison to glycan, protein and DNA due to its inherent instability and lack of effective methods to introduce bioorthogonal reactive functionalities (e.g. azide) into RNA. Here we report the development of a simple and modular posttranscriptional chemical labeling and imaging technique for RNA by using a novel toolbox comprised of azide-modified UTP analogs. These analogs facilitate the enzymatic incorporation of azide groups into RNA, which can be posttranscriptionally labeled with a variety of probes by click and Staudinger reactions. Importantly, we show for the first time the specific incorporation of azide groups into cellular RNA by endogenous RNA polymerases, which enabled the imaging of newly transcribing RNA in fixed and in live cells by click reactions. This labeling method is practical and provides a new platform to study RNA in vitro and in cells.

Item Type:	Article
Additional Information:	This is Open access article for full text click on the weblink
Subjects:	Cancer Biology
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	04 Dec 2015 10:16
Last Modified:	19 Feb 2016 05:43
URI:	http://nccs.sciencecentral.in/id/eprint/214

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