Savanur , M.A. and Eligar, S.M. and Pujari, R. and Chen, C. and Mahajan, P. and Borges, A. and Shastry, P. and Ingle , A. and Kalraiya, R.D. and Swamy , B.M. and Rhodes , J.M. and Yu, L.G. and Inamdar , S.R. (2014) Sclerotium rolfsii lectin induces stronger inhibition of proliferation in human breast cancer cells than normal human mammary epithelial cells by induction. PLoS One , 9 (11). e110107.

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Abstract

Sclerotium rolfsii lectin (SRL) isolated from the phytopathogenic fungus Sclerotium rolfsii has exquisite binding specificity towards O-linked, Thomsen-Freidenreich (Galβ1-3GalNAcα1-Ser/Thr, TF) associated glycans. This study investigated the influence of SRL on proliferation of human breast cancer cells (MCF-7 and ZR-75), non-tumorigenic breast epithelial cells (MCF-10A) and normal mammary epithelial cells (HMECs). SRL caused marked, dose-dependent, inhibition of proliferation of MCF-7 and ZR-75 cells but only weak inhibition of proliferation of non-tumorigenic MCF-10A and HMEC cells. The inhibitory effect of SRL on cancer cell proliferation was shown to be a consequence of SRL cell surface binding and subsequent induction of cellular apoptosis, an effect that was largely prevented by the presence of inhibitors against caspases -3, -8, or -9. Lectin histochemistry using biotin-labelled SRL showed little binding of SRL to normal human breast tissue but intense binding to cancerous tissues. In conclusion, SRL inhibits the growth of human breast cancer cells via induction of cell apoptosis but has substantially less effect on normal epithelial cells. As a lectin that binds specifically to a cancer-associated glycan, has potential to be developed as an anti-cancer agent.

Item Type:	Article
Additional Information:	THIS IS OPEN ACCESS ARTICLE FOR FULL TEXT CLICK ABOVE WEBLINK
Subjects:	Cancer Biology
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	30 May 2016 10:07
Last Modified:	30 May 2016 10:07
URI:	http://nccs.sciencecentral.in/id/eprint/276

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