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Arora , G. and Sajid , A. and Virmani , R. and Singhal A, A. and Santosh Kumar , C.M. and Dhasmana , N. and Khanna , T. and Maji , A. and Misra , R. and Molle, V. and Becher , D. and Ulf Gerth, U and Mande , S.C. and Singh , Y. (2017) Ser/Thr protein kinase PrkC-mediated regulation of GroEL is critical for biofilm formation in Bacillus anthracis. BPJ Biofilms and Microbiomes . ISSN doi:10.1038/s41522-017-0015-4
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5.Dr. Mande S.C. (NPG-Biofilm & Micro) open access.pdf Download (600Kb) | Preview |
Abstract
PrkC is a conserved Ser/Thr protein kinase encoded in Bacillus anthracis genome. PrkC is shown to be important for B. anthracis pathogenesis, but little is known about its other functions and phosphorylated substrates. Systemic analyses indicate the compelling role of PrkC in phosphorylating multiple substrates, including the essential chaperone GroEL. Through mass spectrometry, we identified that PrkC phosphorylates GroEL on six threonine residues that are distributed in three canonical regions. Phosphorylation facilitates the oligomerization of GroEL to the physiologically active tetradecameric state and increases its affinity toward the co-chaperone GroES. Deletion of prkC in B. anthracis abrogates its ability to form biofilm. Overexpression of native GroEL recovers the biofilm-forming ability of prkC deletion strain. Similar overexpression of GroEL phosphorylation site mutants (Thr to Ala) does not augment biofilm formation. Further analyses indicate the phosphorylation of GroEL in diverse bacterial species. Thus, our results suggest that PrkC regulates biofilm formation by modulating the GroEL activity in a phosphorylation-dependent manner. The study deciphers the molecular signaling events that are important for biofilm formation in B. anthracis.
| Item Type: | Article |
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| Additional Information: | This is Open Access Article |
| Subjects: | Structural Biology |
| Depositing User: | Mr. Rameshwar Nema |
| Date Deposited: | 24 Apr 2017 11:12 |
| Last Modified: | 02 Mar 2021 11:04 |
| URI: | http://nccs.sciencecentral.in/id/eprint/420 |
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