Walke , G.R. and Ranade , D.S. and Ramteke , S.N. and Rapole , S. and Satriano , C. and Rizzarell, E. and Tomaselli, G.A. and Trusso Sfrazzetto, G. and Kulkarni PP. , P.P. Fluorescent Copper Probe Inhibiting Aβ1-16-Copper(II)-Catalyzed Intracellular Reactive Oxygen Species Production. Inorganic Chemistry.

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Abstract

A fluorescent copper probe, OBEP-CS1, inhibits amyloid β (Aβ) peptide−copper-catalyzed intracellular reactive oxygen species (ROS) production. OBEP-CS1 in combination with CellROX green was used for the simultaneous monitoring of Aβ peptide-associated intracellular copper redistribution and subsequent changes in ROS production in SH-SY5Y cells.A variety of fluorescent probes are proposed to monitor the intracellular copper content. So far, none of the probes have been evaluated for their potential to inhibit copper-associated intracellular oxidative stress. Herein, we studied the ability of a fluorescent copper probe, OBEP-CS1, to inhibit intracellular oxidative stress associated with an amyloid β (Aβ) peptide–copper complex. The data showed that OBEP-CS1 completely inhibits the copper-catalyzed oxidation as well as decarboxylation/deamination of Aβ1–16. Moreover, the cell imaging experiments confirmed that OBEP-CS1 can inhibit Aβ-CuII-catalyzed reactive oxygen species production in SH-SY5Y cells. We also demonstrated that Aβ1–16 peptide can bind intracellular copper and thereby exert oxidative stress.

Item Type:	Article
Subjects:	Bioinformatics and Proteomics
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	09 May 2017 11:30
Last Modified:	09 May 2017 11:30
URI:	http://nccs.sciencecentral.in/id/eprint/426

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