Dey , C. and Narayan, G. and Krishna Kumar, H. and Borgohain, M.P. and Lenka, N. and Thummer, R.P. (2016) Thummer. Cell-Penetrating Peptides as a Tool to Deliver Biologically Active Recombinant Proteins to Generate Transgene-Free Induced Pluripotent Stem Cells. Stud Stem Cells Res Ther 3(1): 006-015., 3 (1). 006-015.

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Abstract

Delivery of biologically active recombinant proteins using cell-penetrating peptides is a useful tool for transduction of molecular cargo into cells. This protein transduction technology is useful to understand the biological function of a speci fi c protein of interest. Hence, it has been recently employed by several groups to understand the molecular functions of stem cell-speci fi c transcription factors in pluripotent stem cells. Pluripotent stem cells have an inde fi nite self-renewal capacity and can be directed to differentiate into any desired cell type of an adult organism. The groundbreaking discovery of induced pluripotent stem (iPS) cells has revolutionized the fi eld of stem cell research due to its immense potential in in vitro disease modeling, drug screening and regenerative medicine. Although, virus-based gene delivery approaches commonly used to generate iPS cells are robust and highly e ffi cient, however, these methods involve permanent genetic modi fi cations due to viral integrations leading to malignant transformation. Therefore, a protein-based approach to deliver biologically active recombinant proteins in somatic cells to generate iPS cells is safe and will improve the prospects of these cells from bench-to-bedside. This review provides an overview of protein-based somatic cell reprogramming to generate transgene-free iPS cells and gives a glimpse of the bottlenecks associated with this technology.

Item Type:	Article
Subjects:	Stem Cell Biology
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	29 Nov 2017 05:45
Last Modified:	02 Mar 2021 11:36
URI:	http://nccs.sciencecentral.in/id/eprint/463

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