Pendharkar, N. and Dhali, S. and Abhang, S. (2018) A Novel Strategy to InvestigateTissue-Secreted Tumor Microenvironmental Proteins in Serum toward Development of Breast Cancer Early Diagnosis Biomarker Signature. PROTEOMICS - Clinical Applications .

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Abstract

The study aims to discover and identify early grade diagnosismarkers in tumor microenvironment and investigates their expression inserum.Experimental design: 2D fluorescence difference gel electrophoresis(2D-DIGE), a gel-based proteomics platform is used for tissue profiling andidentifies deregulated proteins by matrix-assisted laserdesorption/ionization-time-of-flight mass spectrometry. Western blotvalidation for statistically significant six different proteins is performed in anindependent cohort of participant serum.Results: Total 67 nonredundant tissue proteins are identified out of 230protein spots obtained through Marker selection tool of EDA. GELS, DAPLE,IQCC, CATD, A1AT, and HS90B are selected for validation and found to beupregulated in early grade serum samples. Pathway analysis performedthrough PANTHER and DAVID indicates that apoptosis, CCKR, EGF, FAS,FGF, Wnt, p13 kinase, and p53 signaling pathways are expressed specificallyduring or early onset of grade I cancer lesions.Conclusions and clinical relevance: GELS, DAPLE, HS90B, A1AT, IQCC, andCATD may be promising potential serum biomarker signature for diagnosis ofearly grade breast cancer. This panel also contributes to better understandingof molecular mechanisms involved in inceptive stage of breast cancer.

Item Type:	Article
Depositing User:	Mr. Rameshwar Nema
Date Deposited:	13 Feb 2020 08:56
Last Modified:	13 Feb 2020 08:56
URI:	http://nccs.sciencecentral.in/id/eprint/636

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